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Mutational analysis by next-generation (a,c) and Sanger sequencing (b,d). PDGFRA c.1675_1680del p.Trp559_Arg560del (A and B) and MLH1 c.1572 G > C p.Met524Ile gene alterations (c and d) were identified. Note that red and blue colors in NGS sequence indicate forward and revers reads, respectively.
