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. 2021 May 28;8:643504. doi: 10.3389/fmolb.2021.643504

FIGURE 7.

FIGURE 7

Confirmation of the expression of circRNAs and miRNAs using RNA-sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). CircRNAs and miRNAs were validated by RNA-sequencing (CRSwNP(n = 6); control subjects (n = 3) (A); and qRT-PCR in an independent cohort of five CRSwNP subjects and five control subjects (B). Each sample was detected in triplicate. GAPDH was used as the reference gene for circRNAs, and hsa-miR-16 was used as the reference gene for miRNAs. The relative expression levels of hsa-circ-0031593, hsa-circ-0031594, hsa-circ-0109623, hsa-circ-0000736, hsa-miR-132-3p, hsa-miR-145-5p, hsa-miR-146a-5p, hsa-miR-125a-5p, and hsa-miR-27b-3p by qRT-PCR. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.