Figure 3.

Adipsin deficiency has compensatory activation of C3 without effect on lipid profile. A–D: Three-month-old male DKO and Ldlr^−/− mice were fed the WTD for 14 wk. Plasma was collected after overnight (16 h) fasting (n = 12, 12). Plasma triglycerides (TG) (A), nonesterified fatty acids (NEFAs) (B), total cholesterol (C), and HDL cholesterol levels (D). E: Western blotting of adipsin and C3a in plasma from control and DKO mice, using Coomassie Blue staining as the loading control (L.C.). F: quantification of C3a protein level in plasma (n = 6, 6). Not significant (N.S.). ***P < 0.001 by two-tailed Student’s t test. Data are presented as means ± SD. DKO, double knockout; WTD, Western-type diet.