TABLE 1.

GH families of the infant gut microbiota and their identified specific enzymes that have been found to take part in HMO, GOS and FOS degradation. Enzymes are associated with their target and the bacteria from which they have been isolated.

	GH family	Enzyme	EC number	Target	Bacteria	Genea	References
HMO related	GH18	Endo-β-N-acetylglucosaminidase/Endoglycosidase	EC 3.2.1.96	Galβ1-3GlcNAc2 Galβ1-4GlcNAc2	B. longum subsp. infantis ATCC 15697	EndoBI-1	Parc et al., 2015; Karav et al., 2016; Garrido et al., 2018
					B. longum subsp. infantis 157F/SC142	EndoBI-2	Fukuda et al., 2011; Garrido et al., 2018
	GH20	lacto-N-biosidase	EC 3.2.1.140	GlcNAcβ1-3Gal GlcNAcβ1-6Gal	B. bifidum JCM1254	lnbB	Wada et al., 2008
		β-hexosaminidase/β-1,6-N-acetylglucosaminidase	EC 3.2.1.52		B. bifidum JCM1254	BbhI, BbhII	Miwa et al., 2010
			EC. 3.2.1.-		B. longum subsp. longum JCM1217	BLLJ_1391	Honda et al., 2013
					B. longum subsp. infantis ATCC 15697	Blon_0459, Blon_0732, Blon_2355	Garrido et al., 2012; Kavanaugh et al., 2013
	GH29	α-L-fucosidase	EC 3.2.1.51	Fucα1-3Gal Fucα1-4Gal Fucα1-3GlcNAc Fucα1-4GlcNAc	B. longum subsp. infantis ATCC 15697	Blon_0248, Blon_0426, Blon_2336	Sela et al., 2012; Kim et al., 2013
					B. longum subsp. infantis ATCC 15697	Blon_2336	Sela et al., 2012
		α-1,3/1,4-L-fucosidase	EC 3.2.1.111		B. bifidum JCM1254	afcB	Ashida et al., 2009
	GH33	2,3-2,6-a-sialidase	EC 3.2.1.18	Neu5Acα2-3Gal Neu5Acα2-6Gal Neu5Acα2-6GlcNAc	B. longum subsp. infantis ATCC15697	nanH1, nanH2	Sela et al., 2011
					B. bifidum JCM1254	SiaBb2	Kiyohara et al., 2011
					B. longum subsp. infantis ATCC 15697	Blon_2348	Kim et al., 2013
	GH85	Endo-β-N -acetylglucosaminidase/Endoglycosidase	EC 3.2.1.96	Galβ1-3GlcNAc2 Galβ1-4GlcNAc2	B. longum NCC2705 B. longum DJO10A B. breve	EndoBB	Schell et al., 2002; Garrido et al., 2018
	GH95	α-1,2-L-fucosidase	EC 3.2.1.63	Fucα1-2Gal	B. longum subsp. infantis ATCC 15697	Blon_2335	Sela et al., 2012
					B. bifidum JCM1254	afcA	Ashida et al., 2009
	GH112	GNB/LNB phosphorylase	EC 2.4.1.211	Galβ1-3GlcNAc	B. breve UCC2003	lnbP	James et al., 2016
					B. bifidum JCM1254	LnpA1, LnpA2	Nishimoto and Kitaoka, 2007; Nishimoto et al., 2012
	GH136	lacto-N-biosidase	EC 3.2.1.140	GlcNAcβ1-3Gal	B. longum subsp. longum JCM1217	LnbX	Sakurama et al., 2013
HMO and GOS related	GH1	β-1,4-galactosidase	EC 3.2.1.23	Galβ1-4Glc	Putative
	GH2	β-1,4-galactosidase	EC 3.2.1.23	Galβ1-4Glc	B. longum subsp. infantis ATCC15697	Bga2A	Yoshida et al., 2012
					B. longum subsp. infantis ATCC 15697	Blon_2334, Blon_0268	Garrido et al., 2013; Kim et al., 2013
					B. breve UCC2003	lacZ6	James et al., 2016
					B. breve UCC2003	lacZ(2)	O’Connell Motherway et al., 2013
					B. bifidum DSM20215	BIF1, BIF2, BIF3	Møller et al., 2001
					B. bifidum JCM1254	BbgIII	Miwa et al., 2010
					B. bifidum NCIMB4117	BbgI, BbgIII, BbgIV	Goulas et al., 2009
	GH35	β-galactosidase	EC 3.2.1.23	Galβ1-4Glc	Other species
	GH42	β-galactosidase	EC 3.2.1.23	Galβ1-4Glc Galβ1-3Gal Galβ1-4Gal Galβ1-6Gal	B. breve UCC2003	galG, lntA	James et al., 2016
					B. breve UCC2003	galG, gosG	O’Connell Motherway et al., 2013
					B. longum subsp. infantis ATCC15697	Bga42A, Bga42B, Bga42C	Yoshida et al., 2012; Garrido et al., 2013; Viborg et al., 2014
					B. infantis DSM20088	INF1	Møller et al., 2001
					B. longum subsp. infantis ATCC 15697	Blon_2016, Blon_2416	Kim et al., 2013
					B. bifidum NCIMB4117	BbgII	Goulas et al., 2009
GOS related	GH53	Endo-galactanase	EC 3.2.1.89	Galβ1-4Gal	B. breve UCC2003	galA	O’Connell Motherway et al., 2013
FOS related	GH32	β-fructofuranosidase	EC 3.2.1.26	Glcβ1-2Fru	B. breve UCC2003	fosC	Ryan et al., 2005
		β-fructofuranosidase/fructan β-fructosidase	EC 3.2.1.80 EC 3.2.1.26	Fruβ1-2Fru Glcβ1-2Fru	B. longum ATCC 15697	B.longum_l1	Ávila-Fernández et al., 2016 Warchol et al., 2002
		Exo-inulinase	EC 3.2.1.80	Fruβ1-2Fru	B. longum subsp. infantis ATCC 15697	Blon_2056, Blon_0787	Kim et al., 2013
	GH13	Sucrose phosphorylase/inulinase	EC 2.4.1.7	Glcβ1-2Fru	B. longum subsp. infantis ATCC 15697	Blon_0128, Blon_1740, Blon_0282, Blon_2453	Kim et al., 2013

*The enzymes for which the gene name is not provided are recorded by their genetic locus.