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Alkaline Phosphatase (EC 3.1.3.1) With and Without Na+. (A) Rat intestine alkaline phosphatase homodimer assembled as two 54.4 kDa monomers without Na+ (PDB ID: 4KJG), shown with pNPP substrate in each binding site. (B) Alkaline phosphatase 54.8 kDa monomer activity assayed under conditions of Na+ ion (purple) in the absence of K+ (PDB ID: 3MK1), shown with pH 9.8 reaction products p-nitrophenol and inorganic phosphate (Pi).
