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. 2021 May 28;8:631281. doi: 10.3389/fmolb.2021.631281

FIGURE 2.

FIGURE 2

The import of PIP4K2A from a host cell is conserved in other apicomplexa family members. (A). RBC lysate and isolated purified P. berghei lysate were resolved on 10% SDS PAGE and probed with anti-PIP4K2A (left top panel), and anti-GAPDH (left lower panel). Human foreskin fibroblast cell (HFF) lysate and purified toxoplasma parasite lysate were resolved on 10% SDS PAGE and probed with anti- PIP4K2A (right panel) (n = 3). (B) Immunostaining of purified P. berghei using anti-PIP4K2A {ab154585 (in red)} and DAPI (in blue). The parasite is indicated by arrows. (C) Immunostaining of HFFs infected with toxoplasma using anti-PIP4K2A {(sc100406 (in green)} and DAPI (in blue) (n = 3). The arrows indicate the parasite.