FIGURE 3.

RNA binding activity of PIP4K is conserved among the species. (A). Using ClustalW software, multiple sequence alignment was done, and percentage identity was calculated for different PIP4K’s and is presented in a tabular form. (B) Recombinant proteins were induced by 0.5 mM IPTG and purified using nickel chelating resin. Purity was checked on 10% SDS page. (C) Recombinant PIP4K2A from human, D. melanogaster, C. elegans, and D. rerio was incubated with labeled RAD51-short for 1 h at 16°C. RNA binding activity was checked by resolving the complex and only labeled RNA on 1% agarose at 4°C (n = 3).