TABLE 1.
Experimental study design.
| Group | Group ID | Treatment, (number of days) | No. of animals to be sacrificed post wound creation for collection of skin samples | Total no. of animals/group | ||||
|---|---|---|---|---|---|---|---|---|
| Day 0 a | Day 4 a | Day 7 a | Day14 a | Day 21 a | ||||
| 1 | Diabetic control (no treatment) | No treatment (21 days) | 6 | 6 | 6 | 6 | 6 | 30 |
| 2 | Vehicle (diabetic excision wound with gel alone) | Topical, twice daily (21 days) | - | 6 | 6 | 6 | 6 | 24 |
| 3 | SPPE gel [diabetic excision wound with SPPE in gel (5.0 g extract per 100 g gel)] | Topical, twice daily, (21 days) | - | 6 | 6 | 6 | 6 | 24 |
a
All samples for HP analysis, hydroxyproline content, TGF-ß1, VEGF, EGF (ELISA and qPCR (pooled analysis)); NO, NO synthase.