FIGURE 3.

Cell viability on MTX, vitamin C and MTX plus vitamin C‐treated DBTRG cells. (a) DBTRG cells were treated with 10 μM MTX, 5 μM vitamin C or 10 μM MTX plus 5 μM vitamin C. (b) DBTRG cells were treated with 0.1 μM MTX, 5 μM vitamin C or 0.1 μM MTX plus 5 μM vitamin C. (c) DBTRG cells were treated with 0.01 μM MTX, 5 μM vitamin C, or 0.01 μM MTX plus 5 μM vitamin C. Cell viability was determined by MTT assay and calculated as A570 experimental group/A570 control group × 100%. Data were from four independent experiments and presented as mean ± SD. The * represents p < .05, compared with MTX‐treated group