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PARP cleavage and caspase‐3 activation were observed by Western blotting. (a) DBTGR cells were treated with MTX, vitamin C and MTX plus vitamin C. (b) DBTGR cells were treated with MTX, vitamin E, and MTX plus vitamin E. Lane 1: control; lane 2:10 μM MTX; lane 3:0.1 μM MTX; lane 4:5 μM vitamin; lane 5:10 μM MTX plus 5 μM vitamin; lane 6:0.1 μM MTX plus 5 μM vitamin. Tubulin was used as a loading control
