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. Author manuscript; available in PMC: 2021 Jun 11.
Published in final edited form as: Free Radic Biol Med. 2020 Jul 15;158:60–73. doi: 10.1016/j.freeradbiomed.2020.06.027

Fig. 4. Mutations at R301 do not affect nuclease activity of APE1.

Fig. 4.

Purified APE1, APE1-R301K, and APE1-R301F proteins were used in in vitro nuclease activity assays. (a) AP nuclease activity was assessed using the indicated substrate, with a 5’−32P label. Gradient concentrations were used and APE1 cutting efficiency was analyzed (b). A fluorescently labeled substrate was used in (c). Relative fluorescence units (RFU) were detected by Tecan Infinite F200 Pro. APE1 nuclease activity efficiency was quantified in (d).