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. Author manuscript; available in PMC: 2021 Jun 11.
Published in final edited form as: Free Radic Biol Med. 2020 Jul 15;158:60–73. doi: 10.1016/j.freeradbiomed.2020.06.027

Fig. 7. Methylation deficient mutation sensitizes cells to oxidative treatment.

Fig. 7.

(a) A mitochondrial DNA integrity assay was performed using qPCR. PCR products were quantified using a Quant-iT™ PicoGreen™ dsDNA Assay Kit. Survival assay for HEK-293-R301K cells transfected with wild-type APE1-expressing, R301F APE1-expressing, and vector plasmids after gradient H2O2 (b) and menadione treatment (c). (d) A cytochrome c release assay was performed to detect mitochondrial impairment after H2O2 (50 μM) treatment for 2 h. GAPDH, cytosolic marker; COX IV, mitochondrial marker; H3, nucleus marker. (e) Bar chart for cytochrome c release assay. R301K cells showed significantly more cytochrome c in cytosolic fraction comparing to WT (p < 0.001) or R301F (p < 0.001) mutation, respectively.