Figure 2.

Amplification curves for the 69/70 assay. (A) The green color curve shows the amplification of the probe directed to the sequence without deletion (FAM) in a sample of SARS-CoV-2 positive patient; the curve of the probe directed to the sequence that presents the 69/70 deletion remains without signal of amplification. (B) The red curve shows the probe amplification directed to the 69/70 deletion (CFR 610) using the synthetic control containing the 69/70 deletion. In contrast, the probe directed to the sequence that does not present the deletion 69/70 remains without amplification signal.