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. 2021 May 20;11:672562. doi: 10.3389/fcimb.2021.672562

Figure 4.

Figure 4

Amplification curves of the E484K/N501Y Assay. (A) The green curve shows the amplification of the probe directed to the sequence without the E484K mutation (FAM), while the signal by the probe directed to the sequence that presents the mutation remains without amplification signal; (B) the orange curve shows the amplification of the probe directed to the E484K mutation (CFR 610), while the probe directed to the sequence without mutation remains without amplification signal; (C) the blue curve shows the amplification of the probe directed to the sequence without mutation N501Y (HEX), while the probe directed to the sequence with the mutation remains without amplification signal; (D) the purple curve shows the amplification of the probe directed to the mutation N501Y (Quasar670) with the control containing the mutation (C+ Mut484-501-417), while the probe directed to the sequence without the mutation remains without amplification signal.