Table 1.
Oligonucleotide primer sequences used for C. perfringens toxin genes cpa (α-toxin), cpb (β-toxin), cpb2 (β2-toxin), cpe (enterotoxin), etx (ε-toxin), and iap (ι-toxin)
| Toxin/gene | Primer | Oligonucleotide sequence | Fragment length (bp) | References |
|---|---|---|---|---|
| α/cpa | CPALPHATOX1-F | AAGATTTGTAAGGCGCTT | 1167 bp | |
| CPALPHATOX1-R | ATTTCCTGAAATCCACTC | |||
| β/cpb | CPBETATOX1-F | AGGAGGTTTTTTTATGAAG | 1025 bp | |
| CPBETATOX1-R | TCTAAATAGCTGTTACTTTGTG | |||
| β2/cpb2 | P319BETA2 | GAAAGGTAATGGAGAATTATCTTAATGC | 573 bp | |
| P320BETA2 | GCAGAATCAGGATTTTGACCATATACC | |||
| ε/etx | CPETOXIN1-F | AAGTTTAGCAATCGCATC | 961 bp | |
| CPETOXIN1-R | TATTCCTGGTGCCTTAATT | |||
| Enterotoxin/cpe | CPENT1-F | TAACAATTTAAATCCAATGG | 933 bp | |
| CPENT1-R | ATTGAATAAGGGTAATTTCC | |||
| ι/iap | CPIOTA1-F | AATGCCATATCAAAAAATAA | 821 bp | |
| CPIOTA1-R | TTAGCAAATGCACTCATATT |