Skip to main content
. 2021 Jun 11;16(6):e0252895. doi: 10.1371/journal.pone.0252895

Fig 6. Effects of the psychostimulants cocaine, METH and morphine on the expression and translocation of HDAC1 and GCN5 in human primary astrocytes.

Fig 6

Human primary astrocytes were exposed to cocaine (1 μM), METH (10 μM) and morphine (5 μM) either alone or in combination with piracetam (10 μM) for 24 h. Controls were maintained in drug-free medium (without drug exposure). Representative immunostaining images of (A) HDAC1 expression (red) and nuclear staining with DAPI (blue) obtained by confocal microscopy (magnification 100x, scale bar 100 μm). (C) GCN5 expression (red) and nuclear staining with DAPI (blue) obtained by confocal microscopy (magnification 100x). The data are expressed as the mean ± standard error mean of the corrected total cell fluorescence (CTCF) of three independent experiments. Representative graphs depicting immunostaining analysis results showing CTCF values for HDAC1 and GCN5 for all groups in B and D, respectively. N = 3. ****P<0.0001, ***P<0.001, **P<0.01, *P<0.05, NS—nonsignificant.