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. Author manuscript; available in PMC: 2021 Jun 11.
Published in final edited form as: Methods Mol Biol. 2019;1928:1–27. doi: 10.1007/978-1-4939-9027-6_1

Fig. 1. Examples of experimental setups for stable isotope tracer experiments.

Fig. 1

(a) Sample dish configuration for simultaneous 13C6-glucose labeling of four cell lines, including unlabeled replicate plates (#13–16) incubated with 12C-glucose, as well as a blank replicate plate incubated with media but no cells. (b) Sample dish configuration for evaluation of metabolic perturbations induced by two drugs separately or in combination. Note that pilot experiments should be performed to determine the IC50 of each drug as well as the combination of both drugs, which may act antagonistically or synergistically. (c) Sample dish configuration for the comparative labeling of two cell lines with two different tracers, including unlabeled control replicates and the blank plate incubated with media but no cells