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. Author manuscript; available in PMC: 2021 Jun 11.
Published in final edited form as: Methods Mol Biol. 2019;1928:1–27. doi: 10.1007/978-1-4939-9027-6_1

Table 1.

Total metabolite concentrations measured amperometrically (YSI 2950 Bioanalyzer) in non-dialyzed and dialyzed FBS samples as well as laboratory water sources

Standard heat-inactivated FBS
 Dialyzed FBS
 Water sources
Mfg #1, batch #1 Mfg #1, batch #2 Mfg #2, batch #1 Mfg #2, batch #2 Mfg #1, batch #1 Mfg #1, batch #2 Tap H2O, Bethesda, MD, USA ddH2O
d-glucose, mM 7.95 4.74 3.82 2.75 0.05 0.08 0.04 0.00
l-lactate, mM 18.2 8.25 14.44 17.9 0.17 0.18 0.03 0.00
l-glutamine, mM 0.38 0.1 0.65 0.47 0.01 0.01 0.00 0.00
l-glutamate, mM 0.87 0.57 0.67 0.75 0.00 0.00 0.02 0.03
K+, mM 10.7 n/a n/a 10.5 n/a n/a 0.2 0.0
NH4+, mM 2.9 n/a n/a 2.2 n/a n/a 0.01 0.0