FIGURE 2:

Dural prolactin causes female-specific behavioral responses in rats. Facial withdrawal thresholds were measured in female and male rats prior to and following dural injection of either 5 μg (A, C) or 0.5 μg dural PRL (B, D). Two-way ANOVA followed by Bonferroni multiple comparison analysis indicated significant differences between females that received 5 μg PRL (n = 8 PRL) when compared with those that received vehicle (n = 9). No significant responses were seen in males at this dose (n = 8 PRL, 7 vehicle). At the 0.5 μg PRL dose, female mice that received PRL (n = 8) demonstrated significant hypersensitivity when compared with controls (n = 7). No significant effect was found in males (n = 5 PRL, 5 vehicle). Data are represented as means ± SEM. *p < 0.05,***p < 0.001, ****p < 0.0001. See Table S1 for additional results of analysis.