FIGURE 6:
Crosstalk between the CGRP and PRL signaling systems within the dura. Female prolactin receptor CKO mice (n = 5) and their control littermates (n = 4) had mechanical withdrawal thresholds tested prior to and following injection with CGRP (A, B). Female rats had baseline thresholds assessed and received dural injection of 1 pg CGRP (C, D) (n = 9) or 1 pg of CGRP co-injected with 5 μg of delta PRL (n = 9). Co-injection with delta prolactin significantly attenuated CGRP induced responses, as indicated via two-way ANOVA followed by Bonferroni post-hoc analysis. A separate cohort of female rats received injection of either 0.5 μg dural PRL (n = 10) or co-injection of 0.5 μg PRL with 100 ng CGRP8–37 (E, F) (n = 11). Co-injection with CGRP8–37 significantly reduced behavioral responses to dural PRL. Data are represented as means ± SEM. **p < 0.01,****p < 0.0001. See Table S1 for additional results of analysis.