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. 2021 Jun 11;7(24):eabg5554. doi: 10.1126/sciadv.abg5554

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Fig. 2 — (A) The lengths of FD sperm DNA in the comet tails were standardized against the average lengths of the control sperm results for each irradiation level. (B) The brightness of each male pronucleus immunostaining with anti–γ-H2AX antibodies was plotted. (C) Production rate of offspring using x-ray–irradiated fresh or FD sperm derived from the BDF1 strain. (D) The results of the DNA microarray analysis of FD sperm derived from C57BL/6 sperm and oocytes shown as heatmaps. Four neonates derived from fresh spermatozoa (fresh control), intact FD sperm (FD control), 5 Gy–irradiated FD sperm (FD 5 Gy), and FD sperm treated at −95°C for 1 hour (FD –95°C 1h) were used for each heatmap.