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. 2021 Jun 11;7(24):eabf1771. doi: 10.1126/sciadv.abf1771

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Fig. 2 — (A) Schematic of DNA/DNA and DNA/RNA templates used for the indicated dNTPs. Underlined base codes for the incoming nucleotide. Red, RNA; black, DNA. (B to E) Plots showing relative velocity of the indicated deoxyribonucleotide incorporation by Polθ on DNA/RNA and DNA/DNA primer-templates. Red, DNA/RNA; black, DNA/DNA. Data represent mean ± SD; n = 3. (F) Schematic of DNA/RNA and DNA/DNA primer-templates. Underlined base codes for the correct incoming nucleotide. Red, RNA; black, DNA. (G to J) Denaturing gels showing a time course of Polθ primer extension on the indicated DNA/RNA and DNA/DNA primer-templates in the presence of the indicated deoxyribonucleotide (left). Plots showing percent extension over time (right). Red, DNA/RNA; black, DNA/DNA. dTMP.thymidine monophosphate.