Figure 1.
SMIFH2 inhibits BY-2 cell growth. A, Morphology of BY-2 cells after 5-d treatment with different concentrations of SMIFH2. Scale bar, 50 µm. B, Impact of SMIFH2 on cell growth rate. Relative fresh weight of BY-2 cells was measured after 5-d treatment with SMIFH2. Cultures treated with carrier (DMSO) were taken as 100%. C, Representative images of BY-2 cells stained with fluorescein diacetate (FDA; green, alive) and FM4-64 (purple, dead) from cultures treated for 5 d with DMSO or SMIFH2. Scale bar, 100 µm. D, Viability of BY-2 cells after treatment with DMSO or SMIFH2 for 5 d. Error bars in B and D show standard deviation of three independent replicates containing three technical repeats (n = 3). Asterisks denote samples that are significantly different from the DMSO-treated control (t test P < 0.05).