Figure 4.
Effects of SMIFH2 on phragmoplast microtubule dynamics in BY-2 cells. A, Phragmoplast expansion rate. B and C, Phragmoplast microtubule turnover (t1/2; B) and tubulin immobile fraction (C) in cells treated with SMIFH2, 1 µM propyzamide (PPZ), or 5 µM taxol. D and E, Schematic showing location of the phragmoplast distal zone and midzone regions for measuring microtubule turnover in cells expressing GFP-NtTUA1 (D). Microtubule turnover in different phragmoplast zones (E). F, Ratio of t1/2 in distal zone to midzone. The concentration of SMIFH2 in all experiments was 2.5 µM and treatment time was 3 h. Whiskers denote minimal to maximal range of values. P-values were calculated using Mann–Whitney U test. Different letters denote statistically different values determined by one-way ANOVA. At least five cells were measured for each treatment.