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. 2021 Mar 16;186(2):891–909. doi: 10.1093/plphys/kiab111

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© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of RNAi-mediated knockdown of the CNL1 and 4 in Populus x canescens on the formation of salicinoids. Concentration of salicin (A), salicortin (B), tremulacin (C), salirepin (D), salicin-7-sulfate (E), and salirepin-7-sulfate (F) in leaves of Chrysomela populi-damaged wild-type (WT) and transgenic P. x canescens trees are shown. Compounds were extracted with methanol from freeze-dried leaf material and analyzed using LC–MS/MS and HPLC-UV. Medians ± quartiles, and outliers are shown (n = 4 biological replicates). EV, empty vector; dw, dry weight; ns, not significant. Differences between WT/EV and RNAi knockdown lines were analyzed by Student's t test (ST). Salicin (P = 0.111, t = −1.865, ST); salicortin (P = 0.385, t = −0.936, ST); tremulacin (P = 0.168, t = −1.566, ST); salirepin (P = 0.098, t = 1.961, ST); salicin-7-sulfate (P = 0.242, t = 1.298, ST); salirepin-7-sulfate (P = 0.131, t = 1.748, ST).