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. 2021 Feb 26;186(2):832–835. doi: 10.1093/plphys/kiab102

Figure 2.

Figure 2

Application of AsA increases the production of an antibody that retains binding activity. N. benthamiana leaves were infiltrated with a mixed preparation of Agrobacterium harboring pBYR2HS-PMab2H and pBYR2HS-PMab2L, after which, the leaves were treated with water (Mock) or 200 mM AsA via foliar spray application. At 3 or 4 d postinfiltration, leaves were harvested. Total protein extracts were separated and were transferred to PVDF membranes. The membranes were incubated with anti-mouse IgG(H) or anti-mouse IgG(L) to detect the HC (A) and LC (B) of PMab-2, respectively. C, Native PAGE was used to detect the tetrameric assembly of PMab-2 (two HCs and two LCs). D, Immunoblot analysis with PMab-2 to detect rat PDPN. Soluble proteins from CHO-K1 cells (1) or CHO-K1 expressing rat PDPN (2) were separated on SDS-PAGE. Rat PDPN was detected using PMab-2 derived from hybridoma cells (hybridoma) or N. benthamiana leaves treated without (Mock) or with 200 mM AsA. Total proteins were visualized by CBB staining.