Table 1.
Quantitative growth tolerance of plant FEX sequences compared with native yeast FEX1 and yeast DKO
| Gene | Organism | IC50 (mM) | Fold rescue |
|---|---|---|---|
| p416GPD ScFEX1 | Saccharomyces cerevisiae | 61 ± 7 | 924 |
| p416GPD (empty vector) | None | 0.066 ± 0.003 | |
| At2g41705.1 fully spliced | Arabidopsis thaliana | 47 ± 1 | 712 |
| At2g41705.1 plus intron 2 | Arabidopsis thaliana | 17 ± 2 | 257 |
| At2g41705.1 N-terminal deletion (−17 AA) | Arabidopsis thaliana | 42 ± 9 | 636 |
| At2g41705.1 N-terminal deletion (−129 AA) | Arabidopsis thaliana | 37 ± 5 | 560 |
| At2g41705.1 N186A | Arabidopsis thaliana | 62 ± 16 | 939 |
| At2g41705.1 N373A | Arabidopsis thaliana | 0.018 ± 0.011 | |
| MT248988 | Arabidopsis halleri | 69 ± 9 | 1,045 |
| Sevir.9G321900.1 | Setaria viridis | 58 ± 5 | 879 |
| Niben101Scf02429 | Nicotiana benthamiana | 56 ± 5 | 848 |
| GEFQ01081211.1 | Camellia sinensis | 25 ± 1 | 379 |
| Scaffold_631.172 | Coffea arabica | 71 ± 3 | 1,076 |
| PGSC0003DMT400031487 | Solanum tuberosum | 65 ± 6 | 985 |
| Lsat_1_v5_gn_3_20261.1 | Lactuca sativa | 74 ± 6 | 1,121 |
| GSVIVT01008915001 | Vitis vinifera | 25 ± 7 | 379 |
| Pp3c27_680V3.1 | Physcomitrium patens | 33 ± 1 | 500 |