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. 2021 May 11;10:e63668. doi: 10.7554/eLife.63668

Figure 1. Simple and complex spike activity develops differently between ZebrinII-related Purkinje cell populations.

(A1) Schematic illustration of single unit cerebellar extracellular recordings in Purkinje cells of awake head-fixed mice (left). Schematic representation of a cerebellar coronal section and respective image showing lobules VI, VIII, IX and X, Crus I, Crus II, Paramedian, and Copula Pyramidis. Cerebellar coronal section with PCs labeled with Aldolase C (green). Inset showing recording site marked with biocytin (red) in lobule IX (right). (A2) Example trace of in vivo P30-59 ZebrinII– Purkinje cell recording identified by its hallmark feature, the occurrence of complex spikes (asterisks) and simple spikes. (B1) Example traces of extracellular Zebrin– and Zebrin+ Purkinje cell recordings at P12-17 with clearly distinguishable simple spikes and complex spikes (asterisks). (B2) Photomicrographs of coronal sections with examples of ZebrinII+ (left) and ZebrinII– (right) P12-17 Purkinje cells in lobule X, Paramedian, and Simplex, respectively. Purkinje cells are labeled with Aldolase C (green). Insets showing recording sites marked with biocytin (red) in lobule X, Paramedian (left) and Simplex (right). (C1) Purkinje cell simple spike firing rate, (C2) coefficient of variation 2 (CV2) for simple spikes, (D1) complex spike firing rate and (D2) Climbing fiber pause recorded in vivo for ZebrinII– Purkinje cells (gray) and ZebrinII+ Purkinje cells (purple) in four age groups. Age groups: P12-17, P18-29, P30-59, and >P60. Error bars represent SEM., for values see Supplementary file 1. * denotes p<0.05, **p<0.001 and ***p<0.0001. Scale bars = (A1) 100 µm (left), 500 µm (right), and (B2) 500 µm.

Figure 1—source data 1. Purkinje cell in vivo source data 1.

Figure 1.

Figure 1—figure supplement 1. In vivo extracellular recordings of simple and complex spike activity in different ZebrinII-related Purkinje cell populations during development.

Figure 1—figure supplement 1.

(A1) Example trace of a young P12 Purkinje cell recording identified by its hallmark feature, the occurrence of complex spikes (asterisk) and simple spikes (left). Inset depicting the complex spike (asterisk) following a climbing fiber pause with clear visible spikelets (right). (A2) Photomicrographs of coronal sections with examples of P18-29 ZebrinII–, P30-59 ZebrinII+, >P60 ZebrinII+, and ZebrinII– Purkinje cells labeled with Aldolase C (green). Insets showing recording sites marked with biocytin (red) in lobules IX IV/V and Paramedian. (A3) Example traces of ZebrinII– and ZebrinII+ Purkinje cells extracellular recordings at >P60 ZebrinII+ an ZebrinII–, P18-29 ZebrinII+, and P30-59 ZebrinII– with clearly distinguishable simple spikes and complex spikes (asterisks). (B1) Purkinje cell coefficient of variation (CV) for simple spikes, (B2) regularity index for simple spikes, (C1) coefficient of variation 2 (CV2) for complex spikes and (C2) regularity index for complex spikes recorded in vivo for ZebrinII– Purkinje cells (gray) and ZebrinII+ Purkinje cells (purple) in four age groups. Age groups: P12-17, P18-29, P30-59, and >P60. Error bars represent SEM., for values see Supplementary file 1. * denotes p<0.05, **p<0.001 and ***p<0.0001. Scale bars = (A2) 500 µm (top left, top right, bottom right) and 250 µm (bottom left).
Figure 1—figure supplement 2. Climbing fiber pause is longer than inter-simple spike-interval in the majority of the recorded cells.

Figure 1—figure supplement 2.

(A) Scatterplot of the log mean simple spike-interval versus the log climbing fiber (CF) pause of all cells recorded in four age groups (blue: P12-17; green: P18-29; red: P30-59 and purple: P>60). The diagonal black line represents the reference line where the climbing fiber pause is equally long as the simple spike-interval.
Figure 1—figure supplement 3. In vivo extracellular recordings of simple spike activity distributed by lobules and cerebellar regions during development.

Figure 1—figure supplement 3.

Purkinje cell simple spike firing rate in vivo for Purkinje cells in all lobules at (A1) >P60, (A2) P30-59, (A3) P18-29 and (A4) P12-17. Purkinje cell simple spike firing rate in vivo for (B1) ZebrinII– Purkinje cells in lobules I to V and ZebrinII– Purkinje cells in the hemispheres and for (B2) ZebrinII+ Purkinje cells in the nodulus and ZebrinII+ Purkinje cells in the floculus in four age groups. Error bars represent SEM., for values see Supplementary file 1. * denotes p<0.05, **p<0.001, and ***p<0.0001.
Figure 1—figure supplement 4. Overview with color-coded simple spike and complex spike rate for all identified ZebrinII– and ZebrinII+ Purkinje cells.

Figure 1—figure supplement 4.

(A) P12-P17 Purkinje cell recording location with simple spike rate and (B) P12-P17 Purkinje cell recording location with complex spike rate.
© 2007, John Wiley & Sons
The schematic drawing in panels A and B is redrawn from Figure 3 from Sugihara and Quy, 2007b, with permission from John Wiley & Sons, Inc. It is not covered by the CC-BY 4.0 licence and further reproduction of this panel would need permission from the copyright holder.
Figure 1—figure supplement 5. Overview with color-coded simple spike coefficient of variation 2 (CV2) and coefficient of variation (CV) for all identified ZebrinII– and ZebrinII+ cells.

Figure 1—figure supplement 5.

(A) P12-P17 Purkinje cell recording location with simple spike coefficient of variation (CV2) and (B) P12-P17 Purkinje cell recording location with simple spike coefficient of variation (CV).
© 2007, John Wiley & Sons
The schematic drawing in panels A and B is redrawn from Figure 3 from Sugihara and Quy, 2007b with permission from John Wiley & Sons, Inc. It is not covered by the CC-BY 4.0 licence and further reproduction of this panel would need permission from the copyright holder.