Figure 3. Purkinje cells located in the lobule III display a larger and elaborated dendritic tree.

(A) Photomicrographs of Purkinje cells filled with biocytin in lobule III and X (top panel); and sholl analysis of Purkinje cells located in lobules I/II/III (gray) or IX/X (purple) in six age groups (lower panel). (B₁) Total number of intersections, (B₂) longest dendrite length and (B₃) area analysis for lobules I/II/III Purkinje cells (gray) and lobules IX/X Purkinje cells (purple) in six age groups. Age groups: P3-5, P6-11, P12-17, P18-29, P30-59, and >P60. Error bars represent SEM, for values see Supplementary file 1. * denotes p<0.05, **p<0.001, and ***p<0.0001. Scale bar = (A) 50 µm.

Figure 3—figure supplement 1. Purkinje cell location in the cerebellum within each lobule.

(A) Photomicrographs of Purkinje cells filled with biocytin in lobule III; regions within lobule III (A = apex; B = base; S = sulcus), Scale bar = 500, 200 and 50 µm. (B₁) Percentage of Purkinje cells localized in the apex, sulcus or base at different developmental ages and (B₂) percentage of Purkinje cells localized in the apex, sulcus or base at different developmental ages between A (anterior) and N (nodular) lobules. (C₁) Percentage of Purkinje cells localized in anterior lobules I, II, and III and nodular lobules IX and X at different developmental ages and (C₂) percentage of Purkinje cells localized in lobules I, II, and III and lobules IX and X at different developmental ages between A (anterior) and N (nodular) lobules.