Figure 4. Translocation of climbing fibers occurs earlier in the nodular regions of the cerebellum.

(A) Photomicrographs of lobule III and lobule X regions depicting climbing fiber (VGluT2 staining, cyan) and Purkinje cell (calbindin, red) development in five age groups. (B₁) Molecular layer (ML) thickness, (B₂) VGluT2 puncta per area of region of interest and (B₃) climbing fiber (CF) extension per ML thickness quantification for lobules I/II/III (gray) and lobules IX/X (purple) in five age groups. Age groups: P7, P14, P21, P35, and P60. Error bars represent SEM, for values see Supplementary file 1. * denotes p<0.05, **p<0.001, and ***p<0.0001. Scale bar = (A) 40 µm.