Skip to main content
. 2021 May 11;10:e63668. doi: 10.7554/eLife.63668

Figure 5. ZebrinII+ Purkinje cell axons are larger and less dense at all early postnatal ages.

(A) Photomicrographs of cerebellar sections demonstrating sparse labeling of Purkinje cells. Left panels are stained with antibodies against ZebrinII as well as red fluorescent protein (RFP) to label Purkinje cells expressing RFP. Right panels are stained with DAPI and RFP to show examples of Purkinje cells and Purkinje cell axon arbors (top inset and bottom inset, respectively) in sagittal and coronal cerebellar sections (top and bottom right panels, respectively). (B) Photomicrographs of example ZebrinII– and ZebrinII+ axon arbors from P10, P14, and P21 mice. (C) Sholl analysis of all axon arbors at P7, P10, P14, and P21, average crosses are indicated with points and fitted with a smoothed line. (D1-3) Sholl analyses of ZebrinII– and ZebrinII+ groups of axons at P10, P14, and P21, respectively. (E1) Total number of intersections, (E2) longest dendrite length and (E3) axon arbor area analysis for all axons at different ages. (F1) Total number of intersections, (F2) longest dendrite length and (F3) axon arbor area analysis for ZebrinII– (gray) and ZebrinII+ axon arbors (purple) at P10, P14, and P21. Error bars represent SEM., for values see Supplementary file 1. * denotes p<0.05, **p<0.001, and ***p<0.0001. Scale bars = (A) 500 µm in large panels; 20 µm in top inset, 50 µm in bottom inset. (B) 20 µm.

Figure 5—source data 1. Purkinje cell axonal morphological source data 1.

Figure 5.

Figure 5—figure supplement 1. Random distribution of labeled Purkinje cells for axon analysis.

Figure 5—figure supplement 1.

Sparsely labeled Purkinje cells are randomly distributed throughout the cerebellar cortex at P10, P14, and P21 as exemplified by three representative mice at each age. Purkinje cells are labeled with red fluorescent protein (RFP, red) and the structure of the sections are visible with DAPI (blue). A representative coronal section from the anterior and posterior cerebellum are shown for each mouse. White arrowheads indicate Purkinje cells at P10 when dendrites are not as developed. Purkinje cells in coronal sections are visible as planar lines at P14 and P21. Scale bar = 1 mm.