Antibody | Species | Monoclonal/Polyclonal | Dilution | Catalogue Number | Vendor | Conditions |
---|---|---|---|---|---|---|
V5 (D3H8Q) | Rabbit | Monoclonal | 1:2000 | 13202S | Cell Signaling Technology | 4C overnight |
ZBTB11 | Rabbit | Polyclonal | 1:1000 | A303–240A-M | Bethyl Laboratories | 4C overnight |
Beta-Actin | Mouse | Monoclonal | 1:4000 | A5316 | Sigma-Aldrich | 4C overnight |
Goat anti-mouse secondary | Goat | N/A | 1:5000 | 926–32210 | LI-COR | 20C for 1 hour |
Goat anti-rabbit secondary | Goat | N/A | 1:5000 | 926–68021 | LI-COR | 20C for 1 hour |
Goat anti-rabbit HRP | Goat | N/A | 1:10000 | 7074S | Cell Signaling Technology | 20C for 1 hour |