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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nat Biotechnol. 2021 Jan 28;39(6):697–704. doi: 10.1038/s41587-020-00806-2
Gene sgRNA # sgRNA sequence
ASNSD1 1 GCTCACGTCCTACACTTGAG
ASNSD1 2 TTTGGGTGCCAACTGAAGAG
ASNSD1 uORF 1 GCTTAGATCCTCCTTGTGTG
ASNSD1 uORF 2 TAAAGAACAAAAAATTGTGG
chr2–2 N/A GGTGTGCGTATGAAGCAGTGG
COG7 2 TGTTGAAGCCCTAAAACAGG
COG7 1 CTACTACTACAAGTGTCACA
GREP1 1 ACTCAAAATGGCTATAGACC
GREP1 2 AGGCTTTAGAGGGGACATGA
GREP1 3 GCTCAAAATGGCTTTGGACC
HP08474 1 TGTGTTTGAGCCAGGCATGG
HP08474 2 AGTCCCAGCAGCTACTCCGG
RP11–277L2.3 1 CGCCTCCTGGGTTCCAGCAG
RP11–277L2.3 2 GGGACTAGATGGAGCCGAAG
RP11–54A9.1 1 TGGGTCTCCTCACAGAGTGA
RP11–54A9.1 2 TCCTCAGACCAACCAGCTCA
LacZ N/A AACGGCGGATTGACCGTAAT
ZBTB11-AS1 1 GCGGGACTCTGTATTACCAG
ZBTB11-AS1 2 GCGACGCCGGGACCTCATCG
CTD-2270L9.4 1 CGTGAAGGAGTGGATCAATG
CTD-2270L9.4 2 GAACTTGGAGAAGTCCATGG
G083755 1 CCAACAGGTGACCTCAGCAA
G083755 2 GGACCTCTTACATCATGGAA
SF3B1 N/A AAGGGTATCCGCCAACACAG
ZBTB11 1 ACAGGTTGACACCAAAGGAG
ZBTB11 2 GCATATATTCGACTACACAA
OLMALINC 1 ACAGGGCACTGGTCTCCCAA
OLMALINC 2 CAAGGCTGTATATTTCACCT