FIG. 5.

Adult stem cell proliferation is reduced in T3-treated SRC3 knockout tadpoles. (A) EdU labeling for proliferating cells in WT and SRC3^−/− intestine during T3-induced metamorphosis. The intestinal cross-sections from stage 54 tadpoles treated with 10 nM T3 for up to three days were double stained with EdU for cell proliferation (red) and DAPI for DNA (blue). Scale bar indicates 100 μm. (B) The boxed region in (A) is shown at a higher magnification. White arrowheads denote EdU-positive cells. Scale bar indicates 20 μm. (C) Quantification of cell proliferation as detected in (A, B). The EdU-positive areas (red) in the epithelium were quantified with image j software and normalized against the total cellular area in epithelium as determined by DAPI staining. The experiment was repeated twice with similar results. Each bar represents the mean ± SE. *p < 0.05.