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. 2021 Jun 11;12(6):606. doi: 10.1038/s41419-021-03896-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Effect of NETs on the TEER values of the Caco2 epithelial barrier model. B The morphology of the Caco2 monolayer after NETs treatment was assessed using an ordinary optical microscope. Scale bars = 100 μm. C Cell viability after NETs treatment was tested by cell counting kit-8. D Decreased immunofluorescence localization of ZO-1 was observed in the Caco2 epithelial barrier model at 12 h after NETs treatment. Scale bars = 25 μm. E, F Occludin, claudin 1, and caspase3 protein expression were assessed by western blotting. The intensity was quantified using ImageJ software from three independent analyses. G Analysis of apoptosis (AnxV+) in the Caco2 monolayer after culture with NETs for 12 h assessed by flow cytometry. NETs, neutrophil extracellular traps. TEER transepithelial electrical resistance, AnxV annexin V. Data are expressed as the means ± SD. *P < 0.05, **P < 0.01.