Figure 1.

CA1 pyramidal neurons with and without tau pathology. Confocal microscopy pictures obtained after combining the channels acquired separately for LY (green) and phospho-tau AT8 (red), showing (A–F) neurons and (G–J) their basal dendrites, (A–C,G–H) with a soma free of phospho-tau AT8 (SomaTau-) and (D–F,I–J) with phospho-tau AT8 in an intermediate stage of neurofibrillary pathology (SomaTau +). The position of the soma is indicated with an arrow in (B,C) and (E,F). (K) High resolution image of a dendritic segment indicated with a bracket in J. (L) The same representative image as in (K) showing all the spines along the dendrite marked with a white line and pink dots for their insertion points. (M) 3D spatial distribution of all spines insertion points. Scale bar shown in (M) indicates 12 µm in (A–F), 5 µm in (G–J), and 3 µm in (K–M).