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. 2021 Jun 11;12:3564. doi: 10.1038/s41467-021-23666-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a PPI subnetwork of RNAP. Line thickness between proteins (circles) increases with frequency of observed crosslinks (i.e. one, two, three and more crosslinked residue pairs). Colour scheme for RNAP binders: light grey = coelution correlation > 0.5, dark grey = STRING database combined score ≥ 150, black = both of the previous categories. b Elution traces of RNAP (average abundance of its subunits) and selected RNAP binders with their minimal elution correlation coefficient to any RNAP constituent. c Volcano plot showing the affinity enrichment of SPA-tagged YacL, which co-enriches RNAP (orange) and a number of proteins found crosslinked to the RNAP in the YacL affinity-enrichment experiment (violet). d RNAP with bound NusG (PDB 6C6U [10.2210/pdb6c6u/pdb]) and the region of Crosslinking MS-defined accessible interaction space with 14 satisfied restraints for YacL (I-TASSER model, placed for visualisation purposes) highlighted. Crosslinks between YacL and NusG or RNAP are highlighted in blue.