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. 2021 Jun 11;12:3573. doi: 10.1038/s41467-021-23428-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a The binding profile of STL towards O-GalNAc glycans. b The binding profile of PNA towards O-GalNAc glycans. c The binding profile of Jacalin towards O-GalNAc glycans. The x-axis shows glycans, and the y-axis shows relative fluorescence readout using Cy5-streptavidin (1 μg/mL). PPA = APGS(GalNAcα-)TAPP (100 µM); RPAP = TSAPD(GalNAcα-)TRPAP (100 µM); x-T, Thr-linked counterparts of Ser-linked O-glycans; Biotin = biotinylated PEG amine (0.01 mg/mL); hIgG = human IgG (0.1 mg/mL), mIgG = mouse IgG (0.1 mg/mL); M = Marker (0.01 mg/mL Cy3-conjugated anti-Human IgG + 0.01 mg/mL Alexs647-conjugated anti-Human IgG); NC = printing buffer negative control. n = 3 independent replicates. The individual data points are shown as dots. Data are presented as mean values. Error bars represent standard deviation. Source data are provided as a Source Data file.