Table 1.
Treatments tested based on complete 22 factorial design with central point: yields and protein content obtained for different treatments to extract collagen from chicken MSM residue
| Treatment | Ultrasound time (min) | Enzymatic extraction time (h) | Yield on wet basis (%) | Yield on dry basis (%) | Protein level (%) |
|---|---|---|---|---|---|
| T1 | 30 | 24 | 5.10b ± 0.26 | 11.44b ± 0.60 | 88.62a ± 1.58 |
| T2 | 30 | 48 | 6.74a ± 0.27 | 15.11a ± 0.62 | 88.53a ± 2.91 |
| T3 | 0 | 48 | 4.82b ± 0.23 | 10.80b ± 0.51 | 87.05a ± 2.21 |
| T4 | 0 | 24 | 3.92c ± 0.21 | 8.78c ± 0.47 | 88.34a ± 2.49 |
| PC | 15 | 36 | 5.37b ± 0.26 | 12.03b ± 0.58 | 86.32a ± 1.51 |
aAverages within the same column with the same superscript letters do not differ significantly from each other by Tukey’s test(p > 0.05). Values expressed as mean ± standard deviation.T1: 30 min of pre-treatment with ultrasound and 24 h of enzymatic extraction; T2: 30 min of pre-treatment with ultrasound and 48 h of enzymatic extraction; T3: 48 h of enzymatic extraction; T4: 24 h of enzymatic extraction; PC: 15 min of pre-treatment with ultrasound and 36 h of enzymatic extraction