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. 2021 Jun 11;11:12384. doi: 10.1038/s41598-021-91956-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of an infection with multiple strains of B. burgdorferi s.s. by TBDCapSeq. Shown are alignments of TBDCapSeq reads to fragments of (A) 16S-23S rRNA spacer region, (B) dbpA, and (C) ospC from the N40 strain (accession numbers NC_017416, NC_013130, NC_017401, www.ncbi.nlm.nih.gov). The reference N40 sequence for each gene is indicated with red asterisks. Variant nucleotides are indicated with colors. All three alignments contain a mix of two types of sequences, one identical to strain N40 reference sequence and a variant sequence identical to strain B31 reference sequence. Assemblies were performed in Geneious v 10.0.9 (www.geneious.com).