Table 2.
Read enrichment using TBDCapSeq compared to unbiased high-throughput sequencing (UHTS) in experiment 1.
| Sample type | ospA Ct | Normalized reads (Borrelia reads per million total reads) | Fold enrichment of reads with TBDCapSeq over UHTS | |
|---|---|---|---|---|
| TBDCap seq | UHTS | |||
| Replete larval tick | 21.17 | 544,328 | 5707 | 140 |
| Replete larval tick | 22.25 | 323,316 | 2578 | 125 |
| Mouse 1, Heart | 33.62 | 1272 | 0.067 | 1890 |
| Mouse 1, Ear | 31.40 | 20,819 | 7.27 | 2863 |
| Mouse 1, Bladder | 32.62 | 2930 | 0.35 | 8338 |
| Mouse 2, Heart | 34.23 | 4790 | 1.83 | 2615 |
| Mouse 2, Ear | 29.64 | 34,353 | 13 | 2648 |
| Mouse 2, Bladder | 35.49 | 392 | 0.34 | 1133 |
| Mouse 3, Heart | 33.47 | 2009 | 0.062 | 3213 |
| Mouse 3, Ear | 29.48 | 3546 | 1.01 | 3494 |
| Mouse 3, Bladder | NDa | 829 | 0.09 | 9332 |
aND = not done.