Table 3.

Read enrichment using TBDCapSeq compared to unbiased sequencing in experiment 2.

ospA Ct	Sample type	Sequencing type	Total reads	# of reads mapped to B31	% of reads mapped to B31	Normalized reads (Borrelia reads per million total reads)	Fold enrichment
35.15	Heart Tissue- Mouse 1	Unbiased	10,473,737	0	0	0	1603.10
		TBDCAPSEQ	13,922,800	22,319	0.18	1603.10
34.06	Ear Tissue- Mouse 1	Unbiased	11,451,201	22	0	1.921	6550.90
		TBDCAPSEQ	11,160,954	140,453	1.4	12,584.30
33.99	Ankle Tissue- Mouse 1	Unbiased	9,663,953	3	0	0.31	52,212.80
		TBDCAPSEQ	2,454,532	39,729	1.938	16,185.98
ND	Bladder Tissue- Mouse 1	Unbiased	8,675,559	0	0	0	101.3
		TBDCAPSEQ	13,120,042	1329	0.01	101.3
31	Heart Tissue- Mouse 2	Unbiased	8,956,637	32	0	0	46,207.90
		TBDCAPSEQ	12,485,504	576,929	5.06	46,207.90
33.38	Ear Tissue- Mouse 2	Unbiased	10,377,529	35	0	3.37	8804.90
		TBDCAPSEQ	11,411,883	338,619	3.27	29,672.50
30.49	Ankle Tissue- Mouse 2	Unbiased	7,332,169	46	0.001	6.27	13,893
		TBDCAPSEQ	10,915,727	950,856	9.5	87,108.80
36.67	Bladder Tissue- Mouse 2	Unbiased	10,477,826	1	0	0.1	6691
		TBDCAPSEQ	14,041,858	9817	0.08	699.1
30.29	Heart Tissue- Mouse 3	Unbiased	8,959,037	131	0.002	14.62	1137.03
		TBDCAPSEQ	13,331,701	2,141,384	17.46	160,623.50
32.2	Ear Tissue- Mouse 3	Unbiased	8,148,936	37	0.001	4.54	10,938.30
		TBDCAPSEQ	13,089,033	650,000	5.52	49,659.90
32.83	Ankle Tissue- Mouse 3	Unbiased	7,253,259	18	0	2.48	10,517.70
		TBDCAPSEQ	11,386,644	297,007	2.88	26,083.80
36.32	Bladder Tissue- Mouse 3	Unbiased	8,663,429	0	0	0	2466.70
		TBDCAPSEQ	10,812,361	26,671	0.27	2466.70
24.03	Tick (fed larva 1)	Unbiased	7,014,321	7657	0.122	1091.62	68.89
		TBDCAPSEQ	64,314,285	4,836,678	8.129	75,203.79
NDc	Tick (fed larva 2)	Unbiased	8,210,936	0	0	0	0
		TBDCAPSEQ	63,077,105	1335a	0	0
ND	Salmon sperm DNA	Unbiased	8,214,254	0	0	0	0
		TBDCAPSEQ	694,238	31b	0	0

^aAll mapped to 16S and 23S rRNA of Rickettsia buchneri.

^bAll represented non-Borrelia 16S rRNA reads.

^cND = not done.