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. 2021 Jun 11;4:720. doi: 10.1038/s42003-021-02252-5

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig 4 — Stills of live cell microscopy recordings revealing the CHIM-L distribution in epithelial cell lines and primary endothelial cells in comparison to different markers. a Caveolin1 (mCherry) enriched structures partially colocalize with fluorescently labeled CHIM-L (AF488) in MDCK cells. The inset of the merged panel is shown at higher maginfication in the bottom row. b CHIM-L (AF405) colocalizes with the ectopically expressed cholesterol-binding probe mCherry-D4H at the plasma membrane and some membrane invaginations/internal structures in HeLa cells. c Treatment with the NPC1 inhibitor U18666A, which results in cholesterol accumulation in enlarged late endosomes, sequesters CHIM-L (AF405) into these late endosomes that are also stained with mCherry-D4H in primary endothelial cells (HUVEC). d Lipid droplets stained with Bodipy 493 in comparison to CHIM-L (AF647) in HUVEC. e HeLa cells co-incubated with CHIM-L (AF405) and BODIPY-cholesterol as described in Materials and Methods. Note that CHIM-L shows a more profound plasma membrane staining.