Lysis buffer

Reagents	Stock (prepare individually)		Final concentration	Amount (1×)
Tris, pH 7.5	121.14 g	1M	50mM	2.5 mL
NaCl	292.2 g	5M	150 mM	1.5 mL
MgCl2	406.62 g	2M	10 mM	0.25 mL
Glycerol	n/a	100%	10%	5 mL
NP40	n/a	20%	0.1%	0.25 mL
EDTA	186.12 g	0.5M	1 mM	0.1 mL
PMSF	17.419 g	0.1M	1 mM	0.5 mL
Water	1 L	n/a	n/a	39.9 mL
Total	1 L	n/a	n/a	50 mL

Store at 4°C. Store PMSF and protease inhibitor cocktail solution at −20°C and is stable for several months. Add 1×protease inhibitor cocktail just before use.