Resuspension buffer

Reagents	Stock (20×)		Final concentration	Amount (1×)
Tris, pH 7.5	48.5 g	400 mM	50 mM	6.25 mL
Urea	n/a	n/a	4 M	12.01g
Glycerol	n/a	n/a	2.5%	1.25 mL
SDS	n/a	n/a	2%	1 g
Bromophenol blue	n/a	n/a	0.005%	2.5 mg
Water	1 L	n/a	n/a	upto 50 mL
Total	1 L	n/a	n/a	50 mL
PH	n/a	n/a	n/a	7

It is better to use freshly added urea in the solution as solutions of urea can produce reactive cyanate ions upon standing. Buffer without urea can be stored at 4°C to 25°C for more than a year.