Transfer buffer

Reagents	Stock (10×)		Final concentration	Amount (1×)
Tris	30.28 g	250 mM	25 mM	100 mL of Tris, Glycine mix
Glycine	142.63 g	1.9 M	190 mM
Methanol	n/a	n/a	20%	200 mL
Water	1 L	n/a	n/a	700 mL
Total	1 L	n/a	n/a	1 L

Add methanol just before use. Buffer without methanol can be stored at 4°C to 25°C for more than a year.