Tris-buffered saline with Tween 20 (TBS-T) buffer

Reagents	Stock (20×)		Final concentration	Amount (1×)
Tris, pH 7.6	48.5 g	400 mM	20 mM	50 mL of Tris and NaCl mix
NaCl	175.32 g	3 M	150 mM
Tween 20	n/a	n/a	0.1%	1 mL
Water	1 L	n/a	n/a	949 mL
Total	1 L	n/a	n/a	1 L

Buffer can be stored at 4°C to 25°C for more than a year.