Stripping buffers

Reagents	Stock (20×)		Final concentration	Amount (1×)
Tris, pH 6.8	60.57 g	0.5M	62.5 mM	3.75 mL 3 mL
SDS	200 g	20%	2%
β-mercaptoethanol	n/a	14.3M	100 μM	210 μL
Water	n/a	n/a	n/a	upto 30 mL
Total	1 L	n/a	n/a	30 mL

Buffer can be stored at 4°C to 25°C for more than a year. Add β-mercaptoethanol just before use.