REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-HA antibody	https://www.abcam.com	Cat#ab9110; RRID:AB_307019
Anti-Actin antibody	Sigma-Aldrich	Cat#A0480; RRID:AB_476670
Goat Anti-Rabbit IgG H&L	https://www.abcam.com	Cat#ab6721; RRID:AB_955447
Biological samples
AtTRXL1-HA expressing Arabidopsis lines	Pant et. al, 2020; Mysore Lab, Noble Research Institute	N/A
Chemicals, peptides, and recombinant proteins
Methyl-maleimide polyethylene glycol MM(PEG)24	Thermo Fisher Scientific	Cat#22713
4–15% Criterion TGX Stain-Free Protein Gels	Bio-Rad	Cat#5678083
Pierce™ ECL Western Blotting Substrate	Thermo Fisher Scientific	Cat# 32209
10× Tris/Glycine/SDS Running Buffer	Bio-Rad	Cat #1610772
Trichloroacetic acid (TCA)	Sigma	Cat# T6399
Protease inhibitor cocktail	Sigma	Cat#P9599
Blotting Grade Blocker Non Fat Dry Milk	Bio-Rad	Cat #1706404XTU
CuCl2	Sigma-Aldrich	Cat#203149-10G
DTT (dithiothreitol)	Thermo Fisher Scientific	Cat# R0861
E-Z Store & Pour Fixer	MXR, The Imaging Solution Company	Cat# 114511
E-Z Store & Pour Developer	MXR, The Imaging Solution Company	Cat# 103633
Acetone	Sigma-Aldrich	Cat# 179124-500ML
Software and algorithms
ImageJ	Rueden et al., 2017, https://imagej.net/Welcome	N/A
Other
Criterion Vertical Electrophoresis Cell	Bio-Rad	Cat#1656001
Trans-Blot SD Semi-Dry Transfer Cell	Bio-Rad	Cat# #1703940
Nitrocellulose membrane	Bio-Rad	Cat#1620112
X-Ray Film	Thomas Scientific	Cat#1148B77
Konica medical film processor SRX-101A	Konica Corporation	Cat# 1051/1052
Aluminum Push Button X-ray Cassette - 8×10	soyeeproductsny.com	N/A