Table 1.

Affinity analysis of SFU counts triggered by SARS-CoV-2 peptides in PBMC of donors who recovered from PCR-verified SARS- CoV-2 infection (A) and in Pre-Covid Era subjects (B).

A									B
ID.	ORF3a	N	Nsp12	Nsp5	S(A)	S(B)	S-RBD	M	ID.	ORF3a	N	Nsp12	Nsp5	S(A)	S(B)	S-RBD	M
dC1									dP1
dC2									dP2
dC3									dP3
dC4									dP4
dC5									dP5
dC6									dP6
dC7									dP7
dC8									dP8
dC9									dP9
									dP10
									dP11
									dP12
									dP13
									dP14
									dP15
									dP16
									dP17
									dP18

PBMC of 9 subjects with SARS-CoV-2-PCR-verified infection (A) and PBMC from 18 subjects from the Pre-Covid Era (B) where tested in an ELISPOT assay for reactivity to the specified SARS-CoV-2 mega peptide pools. (These peptide pools are closer defined in Supplementary Table 2 ). All peptide pools were tested in 4 serial dilutions on each PBMC sample at 1.5 ug/mL, 0.5 ug/mL, 0.17 ug/mL, and 0.06ug/mL. Affinity levels are color-coded. Red: high affinity, defined as four consecutive peptide dilutions eliciting a positive recall response with SFU counts exceeding 3 SD of the negative peptide pool-based background. Orange: intermediate affinity, defined as three consecutive peptide dilutions eliciting a positive recall response. Yellow: low affinity, with only the two highest peptide concentrations eliciting positive SFU counts. Beige: only the highest concentration of peptide is positive. The raw counts are provided in Supplementary Table 5 .